Supplementary Movie 2

This video presents a real-time dynamic pseudo-color map illustrating the baseline (BS) and tetanic-evoked potentiation (LTP) states within the DG network. This is achieved by stimulating the perforant pathway with a single high-frequency stimulation (HFS). The recording was performed at a 14 kHz sampling frequency using a full-frame 4096-array from an acute hippocampal slice of a 12-week-old mouse. The clip visualizes the localized initiation and network-wide propagation of LTP across DG subregions (ML, GCL, and Hilus), clearly differentiating between baseline and potentiated states. It pinpoints the precise locations of synaptic strengthening within the network and highlights the spatial heterogeneity of LTP effects. This reveals previously inactive zones that exhibit significant strengthening following LTP induction. The video, with a duration of 6 seconds, captures a single evoked synaptic activation lasting 10 ms in each phase (baseline and post-tetanic) at a frame rate of 25 frames per second